rabbit anti pdgfrβ pab  (Bioss)

Journal: Neurologia medico-chirurgica

Article Title: Platelet-derived Growth Factor Activates Pericytes in the Microvessels of Chronic Subdural Hematoma Outer Membranes.

doi: 10.2176/jns-nmc.2023-0079

Figure Lengend Snippet: Fig. 1 (A) The outer membranes of chronic subdural hema- tomas (CSDHs) from 8 patients were homogenized in sample buffer and used for western blot analysis with anti-NG2, anti- N-cadherin, anti-VE-cadherin, anti-Tie-2, anti-endothelial ni- tric oxide synthase (eNOS), and anti-β actin antibodies. Nota- bly, all these molecules could be detected in the outer membranes of CSDHs. Positive controls are shown in the right 4 lanes, indicating that these molecules were correctly detect- ed. Liver lysate, rat liver whole cell lysate; NIH/3T3, embryo fi- broblast cell lysate; Endothelial cell, endothelial cell lysate; A375 cell lysate, malignant melanoma cell lysate. (B) Western blot analysis of the same samples was performed with anti-phosphorylated platelet-derived growth factor receptor-β at Tyr751 (p-PDGFR β at Tyr751), anti-PDGFR-β, and anti-β actin antibodies. p-PDGFR β at Tyr751, the activated form of PDGFR-β, was detected. Negative CNT, rat brain whole ly- sate, was used as a negative control; positive CNT, rat spleen whole lysate, was used as a positive control.

Article Snippet: The phosphorylated PDGFR-β immunoblots were stripped and reprobed with primary polyclonal anti-PDGFR-β antibodies (Cell Signaling Technology) at a dilution of 1:750 overnight at 4°C.

Techniques: Western Blot, Derivative Assay, Negative Control, Positive Control

Journal: Neurologia medico-chirurgica

Article Title: Platelet-derived Growth Factor Activates Pericytes in the Microvessels of Chronic Subdural Hematoma Outer Membranes.

doi: 10.2176/jns-nmc.2023-0079

Figure Lengend Snippet: Fig. 2 (A) Hematoxylin and eosin staining demonstrated that the outer membrane included inflammatory cells, fibroblasts, and microvessels. Ten-micrometer slices were immunostained with polyclonal antibodies against platelet-derived growth factor receptor-β (PDGF receptor-β, B and C), N-cadherin (D and E), and Tie-2 (F and G) using the ABC method. The areas within the rect- angle, labeled in B, D, and F, are shown at high magnification in Panels C, E, and G, respectively. Notably, these molecules are ex- pressed in endothelial cells (C, E, and G, arrows). Immunostaining without primary antibodies is shown (H). Scale bars = 50 μm (A, B, D, F, and H).

Article Snippet: The phosphorylated PDGFR-β immunoblots were stripped and reprobed with primary polyclonal anti-PDGFR-β antibodies (Cell Signaling Technology) at a dilution of 1:750 overnight at 4°C.

Techniques: Staining, Membrane, Derivative Assay, Labeling, Immunostaining